SGK (SGK1, Serum and glucocorticoid-inducible kinase)

Serum and glucocorticoid-inducible kinase (SGK), a serine/threonine kinase, is a close relative of Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock and mineralocorticoids. SGK activation can be accomplished via HGF, PI3K-dependent pathways and by integrin-mediated, PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of antiapoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion (2). SGK is negatively regulated by ubiquitin modification and proteasome degradation (7).\n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nImmunocytochemistry (IF) 1:100 (on overexpressing cells only) \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts