SGK (SGK1, Serum and Glucocorticoid-regulated Protein Kinase)

The catalytic domain of serum and glucocorticoid-regulated protein kinase (SGK) is 54% identical with that of Akt/PKB. Although SGK1 lacks the PtdIns(3,4,5) P3-binding Pleckstrin-homology domain, it retains the residues that are phosphorylated by PDK1 (Thr256 and Ser422). PDK1 activates SGK in vitro by phosphorylating Thr256. Like Akt/PKB, SGK1 preferentially phosphorylates serine and threonine residues that lie in RXRXXS/T motifs. SGK1 can be activated by PI-3 Kinase, which makes it an important signaling molecule in growth factor and insulin dependent signaling pathways. \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot Analysis: 0.5-2ug/ml detects SGK1 in RIPA lysates from A431 and Rat 2 cells. Rat 2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S1010-83 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\nImmunoprecipitation: 4ug Immunoprecipitates SGK1 from 500ug of Rat 2 lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1010-83

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

=Recognizes human SGK1, Mr ~50kD. A protein, competed by the peptide, was also detected in Rat 2 lysates, Mr ~31kD.\nSpecies Crossreactivity: rat. \nSpecies sequence homology: Rat and mouse 13/15aa identical