SGK2 (Serum and glucocorticoid-inducible kinase)

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of antiapoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion (2). SGK is negatively regulated by ubiquitin modification and proteasome degradation (7).\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1010-85T

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10 mM HEPES pH 7.5, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography. \n

References

1. Webster, M.K. et al. (1993) Mol. Cell. Biol. 13, 2031-2040, 2. Kobayashi, T. and Cohen, P. (1999) Biochem. J. 339, 319-328, 3. Park, J. et al. (1999) EMBO J. 18, 3024-3033, 4. Brunet, A. et al. (2001) Mol. Cell. Biol. 21, 952-965, 5. Mikosz, C.A. et al. (2001) J. Biol. Chem. 276, 16649-16654, 6. Hayashi, M. et al. (2001) J. Biol. Chem. 276, 8631-8634, 7. Brickley, D.R. et al. (2002) J. Biol. Chem. 277, 43064-43070.

Additional Info

Recognizes endogenous levels of human total SGK2 protein. Species Crossreactivity: mouse, rat.