SGLT-1 (Sodium Glucose Transporter 1)

The kidneys play a major role in the regulation of glucose levels. Kidneys filter ~180g of glucose per day from the blood. Most of this glucose is reabsorbed back into the blood via the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, sodium glucose co-transporters (SGLT), in the brush-border membrane. Then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672 residues (75kD). Both N- and C-termini are predicted to be extracellular. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG) is a non metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 . ~70-77kD.\nELISA: 1:10,000-1:50,000. Coat ELISA plates with control peptide at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nS1010-86F\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S1010-86A

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Bov, Rabbit, Sheep

Form

Supplied as a liquid, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Hediger, M.A., et al., Nature 330: 1379-1381 (1987). 2. Sileverman, M., et al., BBA 1153: 43-52 (1993). 3. Pajor, A.M., et al., Am. J. Physiol. 32: R489-R495 (1992). 4. Hirayama, E.A., et al., BBA 1103: 37-44 (1992). 5. Hirayama, E.A., et al., Am. J. Physiol. 261: C296-C304 (1991).

Additional Info

Recognizs SGLT-1. This sequence may also be conserved in other transporters. It is not known if S1010-86A will also identify SGLT-1 like, SGLT-2 or SGLT-3 proteins. Species crossreactivity: Rabbit, rat, mouse, human, pig, cat, dog, reptiles and birds. Species sequence homology: Rabbit, bovine and sheep: 100%; equine and rat: 94%; pig: 89%; chicken: 86%; human: 84%. The Other Sequence Homology: mouse SGLT-3; human SGLT-4a/4b: 84%; human SAAT/SGLT-3: 78%; human and rat SGLT-2: 63%.