SGLT-1 (Sodium Glucose Transporter 1)

The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes bya facilitated sugar transporter \n(GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of approx 659-672 residues (75kD). Both N and C termini are predicted to be extracellular. There is approx 60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10ug/ml (ECL) ~70-77kD.\nELISA: 0.5-1ug/ml. Coat ELISA plates with S1010-86F Control peptide at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-86B

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hediger, MA et al (1987) Nature 330, 1379-1381; 2. Sileverman , M et al (1993) BBA 1153, 43-52. 3. Pajor, AM et al (1992) Am. J. Physiol. 32, R489-R495. 4. Hirayama, EA et al (1992) BBA 1103, 37-44. 5. Hirayama et al (1991) Am. J. Physiol. 261, C296-C304. 6. Lane RH 2002 Am J Physiol Regulatory Integrative Comp Physiol 283: R1450-R1460 IGF alters jejunal glucose transporter expression and serum glucose levels in immature rats-IHC. 7. Dieter M 2004 Obes. Res., 12: 862 - 870 Regulation of Glucose Transporter SGLT1 by Ubiquitin Ligase Nedd4-2 and Kinases SGK1, SGK3, and PKB-IHC.

Additional Info

Recognizes mouse SGLT-1. Species sequence homology: rabbit, bovine and sheep-100%, equine, rat-94%, porine-89%, chicken-86%, human-84% SGLT-1. Mouse- SGLT-3, SGLT-4a/4b-84%, human SAAT/SGLT-3-78%, human and rat SGLT-2- 63%.