SGLT-3 (Sodium Glucose Transporter 3)

The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180 g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucose co transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD). Both N and C-termini are predicted to be extracellualr. There is approx 60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL\nELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plate at 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nS1010-87L: SGLT-3, Mouse Control Peptide (Sodium Glucose Transporter 3).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-87L1

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, .1% BSA

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tatabai Nm et al (2001) Toxicol. Appl. Pharamcol. 177, 163-177. 2. Kong CT et al (1993) JBC 268, 1509-1512. 3. Mackenzie B et al (1994) JBC 269, 22488-22491. 4. Dunham I et al (1999) Nature 402, 489-495. 5. Wright E (2001) Am. J. Physiol. Renal Physiol. 280, F10-F18

Additional Info

Recognizes mouse SGLT-3. No significant sequence homology exists with other SGLTs.