SH3RF2 (SH3 Domain-containing RING Finger Protein 2, Putative E3 Ubiquitin-protein Ligase SH3RF2, Heart Protein Phosphatase 1-binding Protein, HEPP1, Protein Phosphatase 1 Regulatory Subunit 39, PPP1R39, POSHER, RING Finger Protein 158, RNF158)

Although the SH3 domain containing ring finger 2 protein (SH3RF2) has been identified as a marker of the nucleus accumbens in the human brain, little is known about this protein. Recent yeast two-hybrid experiments have shown that SH3RF2 associates with protein phosphatase 1 (PP1) and is expressed primarily in heart and testis tissue. Recombinant SH3RF2 enhanced PP1 enzymatic activity and antagonized the ability of phopho-inhibitor-1 or -2 to inhibit PP1, suggesting that SH3RF2 may play a role working with PP1 in cardiac functions.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\n3T3 (NIH) Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146713

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ernst C, Sequeira A, Klempan, et al. Confirmation of region-specific patterns of gene expression in the human brain. Neurogenetics2007; 8:219-24. 2. Chen CY, Lai NS, Yang JJ, et al. FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1). Biochem. Biophys. Res. Commun.2010; 391:698-702.

Additional Info

Recognizes human SH3RF2. Species Crossreactivity: mouse and rat.