SI-CLP (Chitinase Domain-containing Protein 1, Stabilin-1-interacting Chitinase-like Protein, CHID1, GL008, PSEC0104, SB139)

SI-CLP is a 39kD member of the family of Glyco_18-domain-containing proteins. These chitinase-like proteins are involved primarily in Th2-associated disorders and tumor progression. Chitinase-like proteins are soluble mediators of cell differentiation, proliferation, activation, migration, and adherence. Chitinase like protein SI-CLP is a stabilin-1 interacting partner. SI-CLP gene expression was up- regulated by IL-4 and dexamethasone on the mRNA and protein levels in primary human macrophages in parallel with the expression of stabilin-1. The major site of SI-CLP intracellular localization is in Lamp1-positive and secretion commited CD63+ secretory lysosomes. The endocytic/sorting receptor stabilin-1 is a highly specific intracellular sorting receptor for SI-CLP. SI-CLP directly binds to, and colocalizes with stabilin-1. The lysosomal localization of SI-CLP in stabilin-1-negative macrophages, however, indicates that stabilin-1 may not be the only receptor mediating trafficking of newly synthesized SI-CLP. In vivo, this monoclonal antibody recognizes SI-CLP in the cellular fraction of bronchoalveolar lavage specimens obtained from patients with chronic inflammatory disorders of the respiratory tract and in peripheral-blood leukocytes (PBLs) from these patients as well as healthy donors.\n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50\nImmunofluorescence: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7950-56F

Size

100ug

Applications

IF, WB

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Kzhyshkowska, J et al; Novel stabilin-1 interacting chitinase-like protein (SI-CLP) is up-regulated in alternatively activated macrophages and secreted via lysosomal pathway. Blood 2006, 107: 3221.

Additional Info

Recognizes human SI-CLP.