Sialoprotein, Bone (BSP, Bone Sialoprotein 2, BSP2, Cell-binding Sialoprotein, Integrin-binding Sialoprotein)

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry, RIA, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin and frozen): 1:100\nImmunocytochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-36A

Size

10ug

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 50mM Tris, pH 7.2. Reconstitute with 10ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Wuttke M, Muller S, Nitsche P, Paulsson M, Hanisch FG, Maurer P. Structural characterization of human recombinant and bone-derived bone sialoprotein (BSP) Functional implications for cell attachment and hydroxyapatite binding. J Biol Chem 2001. 2. Ibrahim T, Leong I, Sanchez-Sweatman O, Khokha R, Sodek J, Tenenbaum HC et al. Expression of bone sialoprotein and osteopontin in breast cancer bone metastases. Clin Exp Metastasis 2000;18:253-60.

Additional Info

Recognizes human Bone Sialoprotein and recombinant human BSP. There were no cross reactivities obtained with bovine BSP, porcine BSOP, rat BSP, human Osteonectin and human Osteopontin.