Siglec 7 (Sialic Acid-binding Immunoglobulin-like Lectin, CDw328)

Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage. \n\nApplications: \nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:100-1:200; 10ul labels 1x10e6 cells in 100ul.\nImmunohistochemistry (Frozen): 1:100 \nOptimal dilutions to be determined by the researcher.\n\nIHC Positive Control Tissue:\nFrozen tonsil\n\nRecommended Negative Control:\nI1904-78R: IgG1 Murine Negative Control\n\nHybridoma: \nX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1013-60P

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Falco, M. et al. (1999) Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells. J. Exp. Med. 190: 793-801. 2. Nicoll, G. et al. (1999) Identification and characterization of a novel Siglec, Siglec-7, expressed by human natural killer cells and monocytes*. J. Biol. Chem. 274: 34089-34095.

Additional Info

Recognizes human Siglec 7.