Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898) (FITC)

Siglec-H is a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. This monoclonal antibody exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with this antibody in vitro reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S1013-63A

Size

100ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Blasius, A et al; Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 2006, 107: 2474. 2. Blasius, A et al; A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 2004, 103: 4201. 3. Colonna, M et al; Plasmacytoid dendritic cells in immunity. Nat Immunol 2004, 5: 1219.

Additional Info

Recognizes mouse Siglec-H.