Home  >  Products  >  Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898)
Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898)

Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898)

Cat no: S1013-63

Supplier: United States Biological
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Siglec-H is a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. This monoclonal antibody exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with this antibody in vitro reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Inhibition of IFN-alpha secretion by mouse interferon producing cells. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S1013-63
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
References: 1. Blasius, A et al; Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 2006, 107: 2474. 2. Blasius, A et al; A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 2004, 103: 4201. 3. Colonna, M et al; Plasmacytoid dendritic cells in immunity. Nat Immunol 2004, 5: 1219. 4. Seth, S et al; CCR7 Essentially Contributes to the Homing of Plasmacytoid Dendritic Cells to Lymph Nodes under Steady-State As Well As Inflammatory Conditions. J Immunol 2011, 186: 3364.
Additional info: Recognizes mouse Siglec-H.

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