SIGLEC7, CT (H422) (Sialic Acid-binding Ig-like Lectin 7, Siglec-7, QA79 Membrane Protein, Adhesion Inhibitory Receptor Molecule-1, AIRM-1, p75, D-siglec, AIRM1, D-siglec, CDw328, CD328) (PE)

SIGLECs are cell surface proteins of the Ig superfamily. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. A large subgroup of SIGLECs share high homology with SIGLEC3 (CD33) and are localized to 19q13.4. The cDNA for the SLG gene encodes 2 variants, SLG-long (SLGL) and SLG-short (SLGS). The 595aa SLGL protein contains a signal peptide and 2 V-set N-terminal Ig-like domains. The 477aa SLGS protein has a weak signal sequence and, like most SIGLEC3-like SIGLECs, has only 1 V-set N-terminal Ig-like domain. Both variants contain 2 C2-set N-terminal Ig-like domains, a transmembrane domain, and a cytoplasmic tail with a putative ITIM and a putative SLAM-like tyrosine-based motif. The conserved arginine residue thought to be essential for sialic acid binding in other SIGLECs is replaced by a glutamine in SLGS and by a cysteine in SLGL. RT-PCR analysis detected high expression of both variants in spleen and small intestine, and SLGS was highly expressed in adrenal gland and SLGL was highly expressed in bone marrow. \n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1013-60D-PE

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Nicoll, G., et al., Eur. J. Immunol. 33(6):1642-1648 (2003). 2. Alphey, M.S., et al., J. Biol. Chem. 278(5):3372-3377 (2003). 3. Angata, T., et al., Glycobiology 10(4):431-438 (2000). 4. Falco, M., et al., J. Exp. Med. 190(6):793-802 (1999). 5. Nicoll, G., et al., J. Biol. Chem. 274(48):34089-34095 (1999).

Additional Info

Recognizes human SIGLEC7.