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Signal Recognition Particle, 54kD (SRP54)

Cat no: S1013-70

Signal Recognition Particle, 54kD (SRP54)

The signal recognition particle (SRP) is an oligomeric complex that mediates targeting and insertion of the signal sequence of exported proteins into the membrane of the endoplasmic reticulum. SRP consists of a 7S RNA and six protein subunits. 54kD protein (SRP54) is one of these subunits. It is a GTP-binding protein that interacts with the signal sequence when it emerges from the ribosome. The 54kD subunit of the signal recognition particle has a two domain structure: the G-domain that binds GTP and the M-domain that binds the 7s RNA and also binds the signal sequence. The N-terminal 300 residues of SRP54 include the GTP-binding site (G-domain) and are evolutionary related to similar domains in other proteins.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1906 IgY, Chicken Pab Rb x Ch \nI1906-10 IgY, Chicken-HRP Rb x Ch \nI1906-12 IgY, Chicken-FITC Rb x Ch \nI1906-14 IgY, Chicken-AP Rb x Ch\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-70

Size

50ug

Applications

ELISA, WB

Hosts

Ch/Bird

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

Pool, M. R., Stumm, J., Fulga, T.A.; Sinning, I.; Dobberstein, B.: Distinct modes of signal recognition particle interaction with the ribosome. Science 298: 1345-1348, (2002).

Additional Info

Recognizes human Signal Recognition Particle, 54kD. Species Crossreactivity: Mouse and rat.

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