SIRP, alpha1, aa487-503 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion. It plays a general role in different physiological and pathological processes (1,2,5,8).\n\nApplications:\nSutiable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:2000 dilution. Detects bands at 75-110kD may be SHPTP 1 degradation products.\nOptimal dilutions to be determined by researcher.\n\nPositive Control: Whole cell lysate from THP-1 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-87

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by DEAE chromatography.

Isotype

IgG

References

1. Kharitonenkov, A., et al., Nature 386: 181-186 (1997). 2. Fujioka, Y., et al., Mol. Cell Biol. 16: 6887-6899 (1996). 3. Yamao, T., et al., Biochem. Biophys. Res. Commun. 231: 61-67 (1997). 4. Brooke, G.P., et al., Eur. J. Immunol. 28: 1-11 (1998). 5. Ohnishi, H., et al., J. Biol. Chem. 271: 25569-25574 (1996). 6. Ohnishi, H., et al., Genomics 40: 504-506 (1997). 7. Comu, S., et al., J. Neurosci. 17: 8702-8710 (1997). 8. Stofega, M.R., et al., J. Biol. Chem. 273: 7112-7117 (1998).

Additional Info

Recognizes SIRPa1. The sequences of immunogenic peptide differ from those of mouse, rat and bovine by one amino acid.