SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 may serve as both negative or positive regulators in the intracellular signaling pathways. They are critical in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. Recently identified was a novel SHP-2 associated glycosylated receptor-like protein from human, rat, mouse and cattle by several labs and was designated SIRP-1alpha (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6), p84 (7), and MFR (8). SHPS-1 is a member of a new gene family called the signal regulatory proteins of which there are at least fifteen members. SHPS-1 is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR, and src, a specific docking protein for SHP-2 (1,2,5,9). SHPS-1 has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,9). The mRNA of SHPS-1 is predominantly expressed in hematopoietic cells (2,3).\n \nApplications: \nWestern Blot (Colorimetric): 4ug/ml \nOptimal dilutions to be determined by researcher.

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SPECIFICATIONS

Catalog Number

S1013-87F

Size

25ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.2, containing 0.02% sodium azide. This antibody is supplied as an IgG fraction.

P Type

Pab

Purity

Chromatographically purified

Isotype

IgG

References

1. Kharitonenkov, A. Chen, Z., Sures, I., Wang, H., Schilling, J. and Ullrich, A. (1997) \nNature 386: 181-186. \n2. Fujioka, Y. Matozaki, T., Noguchi, T., Iwamatsu, A., Yamao, T., Takahashi, N., Tsuda, \nM., Takada, T. and Kasuga, M. (1996) Mol. Cell. Biol. 16: 6887-6899. \n3. Yamao, T. Matozaki, T., Amano, K., Matsuda, Y., Takahashi, N., Ochi, F., Fujioka, Y. \nand Kasuga, M. (1997) Biochem. Biophys. Res. Commun. 231: 61-67. \n4. Brooke, G.P. Parsons, K.R. and Howard, C.J. (1998) Eur. J. Immunol. 28: 1-11. \n5. Ohnishi, H. Kubota, M., Ohtake, A., Sato, K. and Sano, S.I. (1996) J. Biol. Chem. 271: \n25569-25574. \n6. Ohnishi, H. et al (1997) Genomics 40: 504-506. \n7. Comu, S. Weng, W., Olinsky, S., Ishwad, P., Mi, Z., Hempel, J., Watkins, S., Lagenaur, \nC.F. and Narayanan, V. (1997) J. Neurosci. 17: 8702-8710. \n8. Saginario, C. Sterling, H., Beckers, C., Kobayashi, R., Solimena, M., Ullu, E. and \nVignery, A. (1998) Mol. Cell. Biol. 18: 6213-6223. \n9. Stofega, M.R. Wang, H., Ullrich, A. and Carter-Su, C. (1998) J. Biol. Chem. 273: 7112-\n7117. \n

Additional Info

Detects bands ranging from 80-95kD, corresponding to the many splice variants of SHPS-1 on SDS-PAGE immunoblots, in human cell lysates and in mouse, rat and bovine brain tissue extracts. The antibody specificity is confirmed in specific peptide inhibition immunoblotting studies