Skp1 (S-phase Kinase-associated Protein, p19skp1)

SKP1 (S-phase kinase-associated protein 1) is a core component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase protein complex. It mediates the ubiquitination of proteins involved in cell cycle progression, transcriptional regulation, and signal transduction. Among the components of the SCF complexes, SKP1 is an adaptor between one of the variable F-box proteins and Cullin (1). Independent of its function as a part of the SCF complex, SKP1 also functions in mitotic exit and cytokinesis. A possible contribution to the function of SKP1 in the mitotic exit is its physical interaction with Bfa1, a key negative regulator of the mitotic exit network (2). SKP1 also plays a crucial role in proper chromosomal segregation, which is required for the maintenance of euploidy and suppression of transformation (3).\nApplications: \nSuitable for use in Western Blot, Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000-1:10,000 \nFlow cytometry: 1:20 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S1014-15D

Size

100ul

Applications

FC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Kong H, et al. Mol. Biol. Evol. 21(1):117-128, 2004 2. Kim N, et al. The Journal of Microbiology 44(6):641-648, 2006 3. Piva R, et al. Molecular and Cellular Biology 22(23):8375- 8387, 2002

Additional Info

Recognizes human SKP1. Species crossreactivity: mouse and rat.

Alternative Names

Cyclin A/CDK2-associated protein p19, p19skp1, p19A, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-2, OCP-II, Transcription elongation factor B, SIII