SLC10A1 (Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1)

SLC10A1(SOLUTE CARRIER FAMILY 10 (SODIUM/BILE ACID COTRANSPORTER FAMILY), MEMBER 1),also called NTCP or NTCP1,is found in the basolateral membranes of hepatocytes, which is also involved in the reabsorption of bile acids. The SLC10A1 gene is mapped on 14q24.2.The SLC10A1 gene contains 5 exons and spans about 23 kb. Like other sodium/bile acid transporters, SLC10A1 contains an exoplasmic N terminus, an odd number of transmembrane regions, and a cytoplasmic C terminus. Two amphipathic sequences are critical for intramolecular interactions and for proper trafficking of SLC10A1 to the plasma membrane.Injection of in vitro transcribed NTCP cRNA into Xenopus oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. NTCP-mediated taurocholate uptake in oocytes was inhibited by all major bile acid derivatives and by bromosulfophthalein.\n\nUniProt Number:\nQ14973\n\nGene ID:\nSLC10A1\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145167

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Green, R. M., Ananthanarayanan, M., Suchy, F. J., Beier, D. R. Genetic mapping of the Na(+)-taurocholate cotransporting polypeptide to mouse chromosome 12. Mammalian Genome 9: 598-600, 1998.\n2. Hagenbuch, B., Meier, P. J. Molecular cloning, chromosomal localization, and functional characterization of a human liver Na(+)/bile acid cotransporter. J. Clin. Invest. 93: 1326-1331, 1994.\n3. Hallen, S., Mareninova, O., Branden, M., Sachs, G. Organization of the membrane domain of the human liver sodium/bile acid cotransporter. Biochemistry 41: 7253-7266, 2002.\n

Additional Info

Recognizes human, mouse and rat SLC10A1. No crossreactivity with other proteins.