Smad2 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily are known to be mediated by SMAD proteins. Biological activities mediated by SMADs include cell growth and morphogenesis, development, and immune response. Activation by TGF-b is mediated by Smad2 and Smad3 while activation by bone morphogenetic protein (BMP) is mediated by Smad1 and Smad5. In contrast to the activating effects of these Smad proteins, Smad6 and Smad7, whose expression is also induced by ligand binding, appear to function as negative regulators of TGF-b superfamily signaling pathways.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-53C

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Casellas, R. & Brivanlou, A.H., Developmental Biology 198(1): 1-12 (1998). 2. Takase, M., et al., Biochemical and Biophysical Research Communications 244(1): 26-29 (1998). 3. Hata, A., et al., Genes and Development 12(2): 186-197 (1998). 4. Imamura, T., et al., Nature 389(6651): 622-626 (1997). 5. Nakao, A., et al., Nature 389(6651): 631-635 (1997). 6. Topper, J.N., et al., PNAS 94(17): 9314-9319 (1997). 7. Riggins, G.J., et al., Cancer Research 57(13): 2578-2580 (1997). 8. Massague, J., Annu. Rev. Biochem. 67: 753-791 (1998) Review. 9. Hata, A., et al., Mol. Med. Today 4: 257-262 (1998) Review. 10. Ulloa, L., et al., Nature 397: 710-713 (1999).

Additional Info

Recognizes human Smad2 and can be used to detect the predominant splice form of Smad2. Crossreactivity with Smad3 or with other Smad family members has not been observed. Antibody reactivity was confirmed by Western Blot using lysates derived from Smad2-transfected COS cells. Species Sequence Homology: Mouse (24/27aa); Xenopus (26/27aa).