Smallpox B5R

Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. It consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-58V3

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Massung,R.F., Loparev,V.N., Knight,J.C., Totmenin,A.V., Chizhikov,V.E., Parsons,J.M., Safronov,P.F., Gutorov,V.V., Shchelkunov,S.N. and Esposito,J.J. Terminal region sequence variations in variola virus DNA. Virology 221 (2), 291-300 (1996).

Additional Info

Recognizes Smallpox B5R. Species Crossreactivity: camel, human. Species sequence homology: bovine, monkey, rabbit.