Smarcd1, ID (Smarcd1, Baf60a, D15Kz1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60kD BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, Protein D15KZ1, SWI/SNF complex 60kD subunit) (AP)

Smarcd1 is involved in chromatin remodeling. Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

042035-AP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Guo, G., et al. Dev. Cell 18(4):675-685(2010)\nHo, L., et al. Proc. Natl. Acad. Sci. U.S.A. 106(13):5181-5186(2009)\nLi, S., et al. Cell Metab. 8(2):105-117(2008)\nOh, J., et al. J. Biol. Chem. 283(18):11924-11934(2008)\nValerius, M.T., et al. Gene Expr. Patterns 8(5):297-306(2008)

Additional Info

Mouse

Alternative Names

Smarcd1, Baf60a, D15Kz1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60kD BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, Protein D15KZ1, SWI/SNF complex 60kD subunit