SNAP 25 (E183) pAb

Syntaxins, six of which have been identified, were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF(N-ethylmaleimide-sensitive factor), SNAP 25, SNAPs (soluble NSF attachment proteins) and synap-totagmin. VAMPs (also designated synaptobrevins), including VAMP-1 and VAMP-2, and Synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including (alpha)- and (gamma)-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis bycompeting with SNAP 25 and syntaxins for VAMP binding.

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SPECIFICATIONS

Catalog Number

BS1327

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 150-200 of Human SNAP 25.

Species

SNAP25 (E183) pAb detects endogenous levels of SNAP25 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P60880

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul