SNRPN, NT (SNRPN, HCERN3, SMN, Small nuclear ribonucleoprotein-associated protein N, Sm protein D, Sm protein N, Tissue-specific-splicing protein) (Azide free) (HRP)

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042103-HRP

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Benetatos, L., et al. Leuk. Res. 34(2):148-153(2010)\nKim, S.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (7), 1116-1125 (2008) :\nHorsthemke, B., et al. Am. J. Med. Genet. A 146A (16), 2041-2052 (2008) :\nRunte, M., et al. Hum. Mol. Genet. 10(23):2687-2700(2001)\nKuslich, C.D., et al. Am. J. Hum. Genet. 64(1):70-76(1999)

Additional Info

Human

Alternative Names

SNRPN, HCERN3, SMN, Small nuclear ribonucleoprotein-associated protein N, Sm protein D, Sm protein N, Tissue-specific-splicing protein