SNW1 (SKIP, SNW Domain-containing Protein 1, Nuclear Protein SkiP, Nuclear Receptor Coactivator NCoA-62, Ski-interacting Protein, SKIIP, Bx42, MGC119379)

TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Skeletal Muscle Tissue Lysate and Human Kidney Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145991

Size

100ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Derynck R, Akhurst RJ, and Balmain A. TGF-bsignaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129. 2. Li Y, Turck CM, Teumer JK, et al. Unique sequence, SkiP, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72. 3. Luo K. SkiP and SkiP: negative regulators of TGF-b signaling. Curr. Op. Gen. Dev. 2004; 14:65-70. 4. Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.

Additional Info

Recognizes human SkiP. Species Crossreactivity: mouse and rat.