SQSTM1, CT (p62) Sequestosome-1, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62, EBI3-associated Protein of 60kD, p60, EBIAP, ORCA, OSIL) (PE)

SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1000\nWestern Blot: 1:50-1:100\nImmunohistochemistry: 1:50-1:100\nImmunofluorescence: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S0961-50A-PE

Size

200ul

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Chen, E.Y., et al., Hum. Mol. Genet. 5(5):659-668 (1996). 2.Toniolo, D., et al., Proc. Natl. Acad. Sci. USA 85(3):851-855 (1988). 3.Seibenhener, M.L., et al., Mol. Cell. Biol. 24(18):8055-8068 (2004). 4.Eekhoff, E.W., et al., Arthritis Rheum. 50(5):1650-1654 (2004). 5.Brajenovic, M., et al., J. Biol. Chem. 279(13):12804-12811 (2004). 6.Kuusisto, E., et al., J. Neuropathol. Exp. Neurol. 62(12):1241-1253 (2003). 7.Johnson-Pais, T.L., et al., J. Bone Miner. Res. 18(10):1748-1753 (2003). 8.Satoru Kobayashi, et al. J. Biol. Chem., Jan 2010; 285: 793 - 804. (Applications: WB)(E-pub) 9.Michal Caspi Tal, et al. PNAS, Feb 2009; 10.1073/pnas.0807694106. (Applications: WB)(E-pub) 10.Jeong-Sun Ju, et al. J. Biol. Chem., Oct 2008; 283: 30289 - 30299. (Applications: IF)

Additional Info

Recognizes human SQSTM1.