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SR Proteins (Non-snRNP Splicing Factor)

Cat no: S6570-50

SR Proteins (Non-snRNP Splicing Factor)

SR proteins are family of highly conserved arginine/serine rich, spliceosome associated phosphoproteins essential for metazoan pre-mRNA splicing (3,4). SR proteins appear to act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role, because they can determine alternative splice site usage in vivo and in vitro (5-7); a particularly interesting example being the regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins (8). SR proteins appear to be recruited from nuclear

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SPECIFICATIONS

Catalog Number

S6570-50

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Tuma, R, et al. J. Cell Biol. 122: 767-773 (1993). 2. Neugebauer, KM and Roth, MB Genes and Development 11: 1148-1159 (1997). 3. Tacke R and Manley JL, Proc Soc Exp Biol Med 220: 59-63 (1999). 4. Graveley BR et al., Curr Biol 9: R6-7 (1999). 5. Fu X-D, Nature 365: 82-85 (1993). 6. Zahler AM, et al., Science 260: 219-222 (1993). 7. Caceres JF, Science 265: 1706-1709 (1994). 8. Kanopka A et al., Nature 393: 185-7 (1998). 9. Misteli T et al., Nature 387: 523-527 (1997).

Additional Info

Recognizes Xenopus SR proteins. Reacts with all members of the SR protein family. The epitope is phosphorylation-dependent so care must be taken to avoid dephosphorylation. Species Crossreactivity: human.

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