Src family, phosphorylated (Tyr416)

Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.\n\nApplications:\nSuitable for use in Western Blot.\nOther applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1: 1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRIPA cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S6504-25B

Size

100ul

Applications

WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Src protein-tyrosine kinase structure and regulation, Roskoski, Robert (2004) Biochem. Biophys. Res. Commun., 324: 1155-64 (2004), 2. Molecular interdiction of Src-family kinase signaling in hematopoietic cells, Geahlen, Robert L, et al. (2004) Oncogene, 23: 8024-32 (2004), 3. Role of STATs as downstream signal transducers in Src family kinase-mediated tumorigenesis, Silva, Corinne M (2004) Oncogene, 23:8017-23 (2004); 4. Structure and regulation of Src family kinases, Boggon, Titus J and Eck, Michael J (2004) Oncogene, 23: 7918-27 (2004), 5. The interplay between Src family kinases and receptor tyrosine kinases, Bromann, Paul A, et al (2004) Oncogene, 23:7957-68 (2004)\n

Additional Info

Recognizes vertebrates phospho-Rsk3 (Thr353/Thr356). Sequence homology: mouse.