Src (pp60Src, pp60c-Src)

The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) is important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity. Phosphorylation of Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). \n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1:1000\nImmunohistochemistry: (Paraffin) 1:800 EDTA/TBST IHC Protocol\nImmunocytochemistry: (IF) 1:100\nImmunoprecipitation: 1:50\nFlow Cytometry: 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nMonoclonal antibodies have traditionally been produced in a mouse. Why a mouse? Because, as a host, it is cost effective, easy to manage, and truly a standard in many biotechnology applications.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S6500-06C

Size

100ul

Applications

FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Mab

Purity

Purified

References

(1) Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513

Additional Info

Detects endogenous levels of Src proteins. The antibody may crossreact with other Src family members. The antibody does not cross-react with other non-family proteins. Species Crossreactivity: Hamster, human, monkey, mouse, rat and bovine.