Staphylococcus aureus produces several different types of enterotoxins (SE's). The SE's are short, soluble proteins that are highly stable and resistant to most proteolytic enzymes. They belong to a family of pyrogenic toxins and as such cause an increase in body temperature, immunosuppression and non-specific T-cell proliferation. These activities are referred to as superantigen activity and results from the interaction of SE's with T-cell antigen receptors and the major histocompatibility complex (MHC) on antigen presenting cells. In addition to their superantigen activity, SE's are also emetics (they cause vomiting). The SE's are important in food poisoning. Enterotoxin G is implicated in scarlet fever, toxic shock and bovine mastitis.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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