Stargazin, CT (Ca2 Gamma Subunit of Voltage-gated Calcium Channel, Cacng2)

Stargazin is a 36kD member of the transmembrane AMPAR regulatory proteins (TARP) family, which plays a vital role in the regulation of the trafficking and localisation/targeting of synaptic AMPA (-amino-3-hydroxy-5-methylisoxazole-4-propionate) receptors (AMPARs).\n\nAMPARs are a major group of ionotropic glutamate receptors, highly concentrated at the postsynaptic density (PSD) of excitatory synapses, which require strict regulation for the control of synaptic strength in the brain. Stargazin has been identified as a vital AMPAR-interacting protein which binds to AMPARs via its extracellular domain, whilst interacting with PDZ scaffolding proteins such as PSD-95, via a cytosolic C-terminal PDZ-binding motif.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7968-30B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 10mM Hepes, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cuadra, A.E. et al. (2004) AMPA receptor synaptic targeting regulated by stargazin interactions with the Golgi-resident PDZ protein nPIST. J. Neurosci. 24: 7491-7502. 2. Choi, J. et al. (2002) Phosphorylation of stargazin by protein kinase A regulates its interaction with PSD-95. J. Biol. Chem. 277: 12359-12363.

Additional Info

Recognizes an epitope within the C-terminal region (CT) of rat stargazin. Species sequence homology: mouse