STAT 1, alpha (p91) (Signal Transducer and Activator of Transcription 1, alpha)

Stimulation of responsive cells with IFN a/b induces the formation of a transcription complex termed ISGF3. This complex binds to the interferon-stimulated response element (ISRE) and thereby activates the transcription of responsive genes. The ISGF3 complex consists of tyrosine phosphorylated STAT1a, STAT1b (p84) and STAT2 proteins, as well as a 48kD (p48) DNA binding protein that specifically binds to the IFN-stimulated response element. Formation of this complex and its migration into the nucleus is dependent upon tyrosine phosphorylation of STAT1a/b and STAT2. Stimulation of cells with IFN-g results in tyrosine phosphorylation of STAT1a (p91) but not of STAT2. Phosphorylation of STAT1a results in homodimerization and migration to the nucleus where it binds to the IFN-g activated site (GAS). The STAT1a and STAT1b isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1b lacks 38 carboxy-terminal amino acids. Contained within these 38 terminal amino acids of STAT1a is a critical serine residue (Ser-727) whose phosphorylation is required for maximal transcription induced by IFN-g. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1.0ug/ml \nWestern Blot: 1-2ug/ml\nImmunohistochemistry (FFPE): 0.1-1.0ug/ml. Heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0 is required prior to staining. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nWestern Blot: A431 cell lysate\nImmunohistochemistry (FFPE): Human tonsil and kidney\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7969-60

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1. Levy, D.E., et al; Genes Dev. 2:383

Additional Info

Recognizes human STAT1a (p91) protein and does not recognize the STAT1b (p84) protein. Does not appear to crossreact with other STAT family members. Species crossreactivity: mouse and rat.