STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1)

The signal transducer and activator of transcription (STAT) family of transcription factors is composed of seven family members (STATs 1, 2, 3, 4, 5A, 5B and 6) that are involved in many cell processes (apoptosis, anti-apoptosis, cell differentiation and proliferation) depending upon the cell type. STATs are activated by ligand binding of mostly cytokine receptors, that receptor associating with a Janus kinase (JAK) family member that phosphorylates a STAT protein causing it to translocate to the nucleus, where it can bind to DNA and regulate the transcription of particular genes. STAT1 serine 727 can be phosphorylated by p38, PKC and CaMKII and has been found to be required for maximum transcriptional activity.\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilution to be determined by the researcher. \n\nPositive Control: \nHEK293 cells treated with UV light; 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7969-07A

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Burysek, L., et al. (2002) The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and Janus kinase (JAK)/STAT signaling\npathways. J. Biol. Chem. 277(36):33509-33517. 2. Schindler, C.W. (2002) Series introduction. JAK-STAT signaling in human disease. J. Clin. Invest. 109(9):1133-1137. 3. Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334. 4. Wen, Z. (1995) Maximal activation of transcription by STAT1 and STAT3 requires both tyrosine and serine phosphorylation. Cell 82(2):241-250.

Additional Info

Recognizes human and mouse STAT1 when phosphorylated at Ser727.