STAT 1 (Signal Transducer and Activator of Transcription 1)

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT 1 and 2) involved in IFN-a and IFN-g signal transduction. Since their initial identification, 5 more STAT proteins have been discovered (STAT 3, 4, 5a, 5b and 6). STATs undergo tyrosine phosphorylations in response to growth factor or cytokine signaling (in some cases mediated by JAK kinases [Janus Kinases 1, 2 and 3]), resulting in dimerization and translocation of STAT proteins to the nucleus. Phosphorylation at serine residues in certain STATs (STAT 1a, 3, 4 and 5) has also been reported, and appears to be required for the maximal activation of these proteins. This antibody recognizes proteins of ~91 and ~84kD, identified as STAT 1a and b. STAT 1 participates in a signaling pathway which is initiated by IFN-g.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman Jurkat and HeLa cells, mouse L929 cells and rat thymocytes. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7969-05G

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS 1% BSA, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1,k

References

1. Burysek, L., T. Syrovets, and T. Simmet (2002) The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and Janus kinase (JAK)/STAT signaling pathways. J. Biol. Chem. 277(36):33509-33517. 2. Schindler, C. and J.E. Darnell Jr. (1995) Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu. Rev. Biochem. 64:621-651. 3. Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334. 4. Wen, Z. (1995) Maximal activation of transcription by Stat 1 and Stat 3 requires both tyrosine and serine phosphorylation. Cell 82(2):241-250.

Additional Info

Recognizes human STAT1a. Species Crossreactivity: Mouse, and rat. Other species were not tested.