STAT 2, phosphorylated (Tyr689) (Signal Transducer and Activator of Transcription 2)

STAT (for signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, which are thought to be essential for their selective activity. Seven STAT proteins have been described (STAT1a, STAT1b, STAT2, STAT3, STAT4, STAT5, and STAT6) and range in molecular weight from 84-113kD. Interferon-a promotes phosphorylation of STAT1 and STAT2, which then form heterodimers that interact with the 48kD DNA binding protein ISGF3. This complex moves to the nucleus where it binds to interferon-stimulated response elements and activates transcription of specific genes. In contrast to interferon-a signaling, interferon-g only induces phosphorylation of STAT1a or b. STAT proteins may be involved in the intracellular signaling of many cytokines and growth factors via the activation of gene expression. \n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:2000 detected phosphorylated STAT2 in HeLa whole cell RIPA lysate stimulated with interferon gamma (0.3ng/ml) for 16-20 hours and then treated with interferon alpha (2000 units/ml) for 20 minutes. HeLa whole cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7970-80 (1:200). Proteins were visualized using a goat anti-rabbit (HRP) secondary and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb \nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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