STAT 5, pan (Signal Transducer and Activator of Transcription 5, pan)

STATs were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play central roles in interferon IFNa-and IFNg-regulated gene expression. Following the identification of these first two family members, five additional mammalian STAT proteins have been cloned, and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6. More recently a putative Drosophila STAT protein termed D-STAT or Marelle was described. All STAT proteins share several conserved structural and functional domains. In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding which results in transcriptional activation of distinct target genes. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml \nWestern Blot: 1-3ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S7972-07B

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Ihle, J.N. (1996) Cell 84:331-334. 2. Schindler, C., and Darnell, J.E. Jr. (1995) Ann Rev. Biochem. 64:621-651. 3. Wakao, H., Gouilleux, F., and Groner, B., (1994) EMBO J. 13:2182-2191. 4. Gouilleux, F., Wakao, H., Mundt, M., and Groner, B. (1994) EMBO J. 13:4361-4369. 5. Azam, M., Erdjument-Bromage, H., Kreider, B.L., et al. (1995) EMBO J. 14:1402-1411. 6. Mui, A.L., Wakao, H., Harada, H., O

Additional Info

Reacts specifically with both mouse STAT5a and STAT5b proteins. Can be used to detect both the un-phosphorylated and phosphorylated forms of STAT5. Does not crossreact with other endogenous STAT isoforms. Species Crossreactivity: Human and rat. Lysates Tested: Cell lysates derived from NIH 3T3 cells, A431 cells, MCF-7 cells, HeLa cells, and 293 cells transfected with either a STAT5 expression vector alone or together with a Jak1 expression vector.