STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence (IC) and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-07C2

Size

40ul

Applications

FC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied in liquid 10mM HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369, 2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191, 3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650, 4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861, 5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159, 6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Additional Info

Recognizes human Phospho-Stat5 (Tyr694). Species Crossreactivity: mouse. Species sequence homology: rat, monkey, bovine.