STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)

Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic \nmyelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. \n \nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. \nOther applications not tested.\n\nRecommended Dilution:\nELISA: 1:20,000\nWestern Blot: 1:500-1:2,000\nImmunohistochemistry: 20-40ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-07H1

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1.Engblom,D., Kornfeld,J.W., Schwake,L., Tronche,F., Reimann,A., Beug,H., Hennighausen,L., Moriggl,R. and Schutz,G. (2007) Direct glucocorticoid receptor-Stat5 interaction in hepatocytes controls body size and maturation-related gene expression. Genes Dev. 21 (10), 1157-1162.2.Baugh,J.E. Jr., Floyd,Z.E. and Stephens,J.M. (2007) The modulation of STAT5A/GR complexes during fat cell differentiation and in mature adipocytes. Obesity (Silver Spring) 15 (3), 583-590. 3.Laurence,A., Tato,C.M., Davidson,T.S., Kanno,Y., Chen,Z., Yao,Z., Blank,R.B., Meylan,F., Siegel,R., Hennighausen,L., Shevach,E.M. and O'Shea,J.J. (2007) Interleukin-2 signaling via STAT5 constrains T helper 17 cell generation. Immunity 26 (3), 371-381. 4.Burchill,M.A., Yang,J., Vogtenhuber,C., Blazar,B.R. and Farrar,M.A. (2007) IL-2 receptor beta-dependent STAT5 activation is required for the development of Foxp3+ regulatory T cells. J. Immunol. 178 (1), 280-290.

Additional Info

Recognizes human STAT 5, phosphorylated at Tyr694. Species Sequence Homology: rat.