STAT 5, phosporylated (Tyr699) (Signal Transducer and Activator of Transcription 5)

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-a). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.\n\nApplications: \nSuitable for use in Immunocytochemistry and Western Blot. Other applications not tested.\n\nReommended Dilution:\nImmunocytochemistry: 10ug/ml detected phospho-STAT5 (Y699) in IFNa-treated Daudi cells. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton(R) X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-rabbit IgG in the dark. Between each step, cells were washed with PBS containing BSA.\n\nWestern Blot: 1.0ug/ml\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-07N

Size

100ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a powder lyophilized from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 100ul of sterile PBS containing 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Detects human STAT5A phosphorylated at Y694 and STAT5B phosphorylated at Y699. Does not recognize STAT5A/B unphosphorylated at Y694/Y699.