STAT 5 (Signal Transducer and Activator of Transcription 5)

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation . This phosphorylation is mediated by Src upon erythropoietin stimulation. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Chromatin Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nChromatin Immunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-07L

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369. 2. Wakao, H. et al. (1994) EMBO J. 13, 2182-2191. 3. Okutani, Y. et al. (2001) Oncogene 20, 6643-6650. 4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861. 5. Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159. 6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.

Additional Info

Recognizes endogenous levels of total Stat 5 alpha and beta protein at ~90kD. Species Crossreactivity: human, mouse, monkey and rat.