STAT 5a,b, phosphorylated (Tyr694/Tyr699) (Signal Transducer and Activator of Transcription 5a,b)

STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases. In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT. Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport. STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production. The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000 detected phospho-STAT5A/B in A431 cells stimulated with EGF. The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S7972-13. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.\nOptimal dilutions to be dtermoned by the researcher.\n\nPositive Control: \nIFN-alpha induced HeLa cells, GM-CSF treated TF-1 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-13

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Sheep

Form

Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Darnell, J. E., et al., Science 264: 1415-1421, 1994. 2. David, M., et al., Science 269: 1721-1723, 1995. 3. Kirken, R.A., et al., J. Biol. Chem. 272: 15459-15465, 1997.

Additional Info

Recognizes the phosphorylated tyrosine residues 694 of human STAT5A, 94kD and 699 of STAT5B, 92kD.\nSpecies Crossreactivity: rat, mouse, bovine and sheep.