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STAT 5A, phosphorylated (Ser780) (Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5, Mammary Gland Factor, MGF)

Cat no: S7972-08D

STAT 5A, phosphorylated (Ser780) (Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5, Mammary Gland Factor, MGF)

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. Both Stat5a and Stat5b regulate interleukin-7 induced B-cell precursor expansion. Activation of the protein in myeloma and lymphoma associated with a TEL:JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. Human STAT5A gene is mapped to chromosome 17q11.2.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-08D

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Luo, G. et al. Mol. Endocrinol. 14:114-123 (2000). 2. Leonard, WJ. et al. Annu. Rev. Immunol. 16:293-322 (1998).

Additional Info

Recognizes human STAT5A when phosphorylated at Ser780. Species Crossreactivity: mouse and rat.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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