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STAT3 (Signal transducer and activator of transcription 3, Acute-phase response factor)

Cat no: 030663

STAT3 (Signal transducer and activator of transcription 3, Acute-phase response factor)

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development . Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3a (86kD) and Stat3b (79kD) depend on cell type, ligand exposure or cell maturation stage. It is notable that Stat3b lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nImmunohistochemistry: 1:200-1:1000.\nImmunofluorescence: 1:200-1:1000.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030663

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, NHP

Form

Ascites fluid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. J Mol Graph Model. 2009 Nov;28(4):347-56. \n2. Bone. 2010 Feb;46(2):524-33.

Additional Info

Human, Mouse, Monkey

Alternative Names

APRF; HIES; FLJ20882; MGC16063; STAT3

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