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STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (FITC)

Cat no: S7972-73N-FITC

STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (FITC)

LYK5, also STE20-related Adaptor Protein (STRAD) are members of STE-20 like kinase family that are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. STRAD-LKB1 interaction results in phosphorylation of STRAD and enhanced autophosphorylation of LKB1.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-73N-FITC

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Baas, A.F., et al., EMBO J. 22(12):3062-3072 (2003). 2. Scanlan, M.J., et al., Cancer Immun. 1, 4 (2001).

Additional Info

Recognizes human LYK5.

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