Steroidogenic Factor 1 (SF-1, NR5A1)

Steroidogenic factor (SF-1), is an orphan nuclear receptor that regulates the enzymes that produce steroids and is required for adrenal and gonadal development. SF-1 is important in the regulation of the pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH). SF-1 is also expressed at high levels in the embryonic diencephelon that gives rise to the endocrine hypothalamus. Therefore, SF-1 plays an important role in the development and function of multiple endocrine organs that are essential for reproduction. \n\nSF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunoblot Analysis: 0.5-2ug/ml of this lot detected SF-1 in RIPA lysates from Y1 cells.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-54W

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Ikeda, Y., et al., Mol Endocrinol., 7: 852-860, 1993.\n2. Lynch, J.P., et al., Mol. Endocrinol., 7: 776-786, 1993.\n3. Ingraham, H.A., et al., Genes Dev., 8: 2302-2312, 1994.\n4. Hu, Z., et al., J. Biol. Chem., 272: 14263-14271, 1997.\n5. Parker, K.L., et al., Recent Prog. Horm. Res., 57: 19-36, 2002.\n6. Fowkes, R.C., and Burrin, J.M., J. Endocrinol., 177: 345-350, 2003.\n7. Zhao, L., et al., Mol. Cell. Endocrinol., 215: 89-94, 2004.

Additional Info

Recognizes mouse SF-1, Mr 53kD.