Stk3, CT (Stk3, Mess1, Mst2, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase 3 36kDa subunit, Serine/threonine-protein kinase 3 20kDa subunit)

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042402

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Oh, H.J., et al. Curr. Biol. 20(5):416-422(2010)\nLu, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1437-1442(2010)\nSong, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1431-1436(2010)\nZhou, D., et al. Cancer Cell 16(5):425-438(2009)\nChoi, J., et al. PLoS ONE 4 (11), E8011 (2009) :

Additional Info

Human

Alternative Names

Stk3, Mess1, Mst2, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase 3 36kDa subunit, Serine/threonine-protein kinase 3 20kDa subunit