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Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

Cat no: S7975-11G

Supplier: United States Biological
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Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.03-0.1ug/ml, observed in lysates of cell line K562 on ~160kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7975-11G
Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Hauf S, Roitinger E, Koch B, Dittrich CM, Mechtler K, Peters JM, Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2. PLoS biology 2005 Mar 3(3):e69.
Additional info: Recognizes human Stromal antigen 2. Species sequence homology: Bovine, canine, mouse, porcine and rat.

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