Stromal Interaction Molecule 1 (STIM1)

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++- release-activated Ca++ (CRAC) channels (reviewed in 1). These channels are made up of the pore-forming subunit ORAI1 (2) and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store (3,4). A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a\ncoordinated role in controlling Ca++ entry (5). At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted\nto have no cross-reactivity to STIM2.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2 ug/ml.\nImmunohistochemistry: 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse thymus tissue lysate\nHuman spleen tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S7975-88

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7. 2. Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85. 3. Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 2005; 437:902-5. 4. Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.. Soboloff J, Spassova MA, Hewavitharana T, et al. STIM2 is an inhibitor of STIM1-mediated store-operated Ca2+ entry. Curr. Biol. 2006; 16:1465-70.

Additional Info

Recognizes human STIM1. Species Crossreactivity: mouse and rat.