Syntaxin, a 33kD molecule with carboxy terminal membrane anchor, is a synaptic protein identified by its ability to interact with the synaptic vesicle protein synaptotagmin. It contains one 1 t-SNARE coiled-coil homology domain and has been implicated in docking at synaptic vesicles of presynaptic neurotransmitter release sites.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot ( A-431 lysate, mouse lung lysate): 1:500-1:1000
Western Blot (transfected 293T lysate): 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Immunogen Sequence (without GST): MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVLIAICVSITVVLLAVIIGVTVVG
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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