Substance P Receptor (NK1 Receptor, Neurokinin Receptor)

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma.\nThree tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1:1000 (ECL) A band is seen at approximately 53kD representing human NK-1. The actual molecular weight may vary depending on the tissue used. \nOptimal dilution determined by the researcher. \n\nPositive Control: \nSF9 whole cell membrane extracts\n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8000-51A

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Vigna, S.R., et al, The Journal of Neuroscience. 14(2): 834-845, 1994. 2. Mantyh, P.W., et al,. Science. 278: 275-279, 1997. 3. Mantyh, C.R. et al, . Gastroenterology. 111:1272-1280, 1996. 4. Mantyh, P.W. et al, PNAS. 92: 2622-2626, 1995. 5. Mantyh, P.W., et al, Science. 268: 1629-1632, 1995. 6. Mantyh, P.W. et al, Brain Research. 719: 8-13, 1996. 7. Allen, B.J., et al, . Journal of Neuroscience. 17(15): 5921-5927, 1997.

Additional Info

Recognizes human NK-1. Species Crossreactivity: rat.