Substance P Receptor (NK1 Receptor, Neurokinin Receptor)

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the C-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. \n\nThree tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).\n\nApplications: \nSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry/Immunocytochemistry: 1:1000; Can be used on frozen and fixed tissue sections and permeabilized cells.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8000-51D

Size

100ul

Applications

IHC

Hosts

Rabbit

Reactivities

Mouse, Rat, GP

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.\n

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Mantyh, P.W., et al, Inhibition of Hyperalgesia by Ablation of Lamina I Spinal Neurons Expressing the Substance P Receptor. Science. 278: 275-279, 1997. \n2. Mantyh, C.R. et al, Substance P Activation of Enteric Neurons in Response to Intraluminal Clostridium difficile Toxin A in the Rat Ileum. Gastroenterology. 111:1272-1280, 1996. \n3. Mantyh, P.W. et al, Rapid endocytosis of a G protein-coupled receptor: Substance Pevoked internalization of its receptor in the rat striatum in vivo. PNAS. 92: 2622-2626, 1995. \n4. Mantyh, P.W., et al, Receptor Endocytosis and Dendrite Reshaping in Spinal Neurons After Somatosensory Stimulation. Science. 268: 1629-1632, 1995. \n5. Mantyh, P.W. et al, Differential expression of two isoforms of the neurokinin-1 (substance P) receptor in vivo. Brain Res. 719: 8-13, 1996. \n6. Allen, B.J., et al, Noxious Cutaneous Thermal Stimuli induce a graded release of endogenous substance P in the Spinal Cord: Imaging Peptide Action in vivo. J. of Neuroscience. 17(15): 5921-5927, 1997. \n7. Vigna, S.R., et al, Characterization of Antibodies to the Rat Substance P (NK-1) Receptor and to a Chimeric Substance P Receptor Expressed in Mammalian Cells. The J. of Neuroscience. 14(2): 834-845, 1994

Additional Info

Recognizes rat Substance P Receptor. \nSpecies Crossreactivity: mouse and guinea pig